No, a male employee cannot file a case under Prevention of Sexual Harassment Act at workplace. The act provides only for sexual harassment cases against a woman and not a man. An aggrieved woman under the Act includes all women, irrespective of her age or employment status, whether in the organized or unorganized sectors, public or private and covers clients, customers, and domestic workers as well. It is a gender-biased law tilting towards one gender only.
However, if you witness incidents of assault at workplace by a woman or a man, irrespective of the gender you can file a case under Section 351 of Indian Penal Code(IPC) with the help of best criminal lawyers in India. Assault is any act or gesture or preparation, intentionally committed to threaten the person or direct criminal force upon such a person.